CHEMISTRY 799

CHEMISTRY 785L
BIOCHEMISTRY LABORATORY I
6022 Ward Beecher
MW 1:25 - 4:15

Thomas Kim, 6027 Ward Beecher
Office hours: MW 11:30-12:30; T 9:00-10:00
or by appointment
office phone: 742-7102
email: tdkim@cc.ysu.edu

TEXTBOOK:

R. F. Boyer, Modern Experimental Biochemistry, 2nd ed. 1993, Benjamin/Cummings Publ.

LAB NOTEBOOK:

Laboratory notebooks will be required for the recording and write-up of laboratories this quarter. The notebook shall be a duplicate carbonless type.

GRADING:

While students will perform experiments in pairs, each student will be assessed on an individual basis for his/her lab grade. The grade will be assigned in the following manner:

Attendance and Preparation for Laboratory (25%)

Pre-laboratory exercises will be due at the beginning of each new laboratory to monitor preparation for the lab experiment. Also, lab notebooks will be kept in carbonless duplicate form notebooks. For your lab reports, you should turn in the carbon-copy of the work done during the experiment along with a write up (also done in notebooks), described below.

Lab Reports (60%)

In the lab period following the completion of an experiment, you shall turn in a formal report of the experiment in the following format. The overall length should not exceed two pages (not including additional attachments/data):

1. Title of experiment, Student Name and Lab Partner Name
2. Abstract
This should be a short (approximately 50-75 word) description of the purpose, biochemical principle and the outcome of the experiment)
3. Data
Present data in a final compiled form (descriptive, tabular or as a graph). Strive for clarity and conciseness.
4. Analysis of Results and Conclusions
Briefly discuss the results and what conclusions you can draw from them.
Discuss any difficulties or lack thereof as they pertain to the analysis of your data.

General Technique, Safety and Cleanliness (15%)

LABORATORY SCHEDULE:

Date	Laboratory Title	Source
WEEK I	Check-In
	Expt 2: Analytical Methods for Amino Acid Separation and Identification (Prelab)	Boyer: pp. 229-242

WEEK II	Expt 3: Purification and Characterization of a-Lactalbumin, a Milk Protein (Part A, B & handout)	Boyer: pp. 243-266

WEEK III	Expt 5: Kinetic Analysis of an Enzyme, Tyrosinase	Boyer: pp. 299-322
	Expt 9: Identification of Carbohydrates by Polarimetry	Boyer: pp. 347-362

WEEK IV	Expt 15: Vitamin C Content of Fruits, Vegetables and Other Foods	Boyer: pp. 433-440
	Expt 7: Purification and Characterization of a Lipid Extracted from Nutmeg	Boyer: pp. 323-334

WEEK V	Checkout/Make up Labs

Disclaimers
The materials fees for this laboratory course are used to partially defray the cost of replacing expendable materials and obsolete equipment repairing equipment and obtaining new equipment appropriate for student use in state-of-the-art instruction in chemistry laboratory.

SAFETY REGULATIONS

Never pipette by mouth. Pipet bulbs/pipet-aids are to be used in all cases.
Protective eye ware must be worn by all students. Regular eyeglasses are not an adequate substitute for safety goggles. Also, proper lab coats must be worn at all times in the laboratory
All poisonous and foul-smelling chemicals (phenol, acrylamide, etc.) must be handled under the fume hood. If the hoods are all in use, --wait--, that is certainly better than breathing potentially hazardous compounds.
All spills on benchtops, around balances and within the centrifuge must be cleaned up immediately. Do not leave spilled water on the floor.
Label all samples, reagent bottles and solutions with the chemical identity of the their contents, your name and date of preparation. Unlabeled samples will be discarded on a regular basis. Use bio-hazard warning labels for samples that warrant this caution, but do not misuse these important warnings.
Report all accidents and injuries immediately to your instructor.
Be aware of the toxicity of all chemicals used in the laboratory and the appropriate steps that must be taken in case of accidental exposure. Your prelab preparation should include a survey of reagents to be used in the laboratory along with pertinent safety and handling information.